Journal: Molecular cell

Article Title: The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex.

doi: 10.1016/j.molcel.2024.04.007

Figure Lengend Snippet: Figure 4. Exosome function controls the dynamics of the MYCN/MAX/MXD network (A) Immunoblot of IMR-32 cells stably expressing an shRNA targeting EXOSC10 treated for 72 h with Dox or EtOH as control. ACTB was loading control. Blot is representative of n = 3 independent replicates. (B) Read distribution of MYCN ChIP-Rx, MAX, MNT, and SIN3A cleavage under targets & release using nuclease (CUT&RUN) at the CDK5RAP3 and PHB genes. Cells treated as in (A) (n = 3 for MYCN ChIP-Rx; n = 2 for all C&Rs).

Article Snippet: 3 mg of each, MAX (Proteintech), MNT (Thermo Fisher Scientific), or SIN3A (Novus Biologicals) antibody were added per sample and incubated with shaking (800 rpm) at 4 C overnight.

Techniques: Western Blot, Stable Transfection, Expressing, shRNA, Control

Journal: Journal of Gastroenterology and Hepatology

Article Title: Ileal FXR Knockdown Ameliorates MASLD Progression in Rats via Modulating Bile Acid Metabolism Mediated by Gut Microbiota

doi: 10.1111/jgh.17017

Figure Lengend Snippet: Ileal Nr1h4 knockdown ameliorates hepatic steatosis and inflammation in MASLD rats. (A) Representative immunohistochemical images of FXR in ileum and liver of the indicated groups. Scale bar, 200 μm. (B) The expression levels of Nr1h4 and/or Shp in the ileum and liver of the indicated groups was examined by qPCR. (C) Western blotting was preformed to examine FXR expression in ileum of the indicated groups. (D) The expression levels of Nr1h4 , Shp , and Fgf15 was examined by qPCR in the ileum of the indicated groups. (E) Representative images of HE, Oil Red O, and Sirius red staining of the liver from the indicated group. Statistical chart is shown on the right. Scale bar, 200 μm. (F) Serum levels of ALT, AST, TG, and TC in the indicated groups. (G) Representative immunofluorescence images for F4/80 in the indicated groups. Statistical chart is shown on the right. Scale bar, 200 μm. (H, I) The expression levels of Scd1 , Acc1 , Srebp1c , Fasn , Tnfα , Tgfβ , Il‐1β , and Il‐6 in the ileum of the indicated groups was examined by qPCR. Data are presented as mean ± SD ( n = 4–5 for each group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The sections were subsequently incubated overnight at 4°C with primary antibodies against FXR (1:100; Proteintech, USA) and F4/80 (1:200; CST, USA).

Techniques: Knockdown, Immunohistochemical staining, Expressing, Western Blot, Staining, Immunofluorescence